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A Mitochondrial Protein Hunt

Dec. 18th, 2025 02:11 pm
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One of the constant themes of cell biology, chemical biology, and drug discovery is trying to find out where things are in cells. It isn’t easy! You can’t just pick out a small molecule, a protein, a lipid, a polysaccharide or what have you and chase it around a living cell with some sort of microscope, because there’s generally either no good way to detect such things specifically or certainly no way to do it on the small scale required. It’s also difficult to just reach into cells and pluck out specific structures or regions and comprehensively inventory them, although over the years we’ve become better and better at it - for some specific cellular fractions and some specific classes of compounds, to be sure.

But the most widely uses methods continue to rely on imaging of cells in the presence of some sort of fluorescent system that causes things to light up so we can finally see them. As the practitioners of these arts well know, this is a tricky business, because it’s all too easy to change the properties of what you’re trying to study by hanging a nice bright fluorophore group off of it. Function, transport, eventual localization, half-life and stability: all of these can be messed up in unpredictable ways, so every time you get fluorescent labeling data it’s good scientific discipline to ask yourself how it might be misleading, and what you can do to cross-check the numbers.

There are a lot of ingenious ways to deal with these problems, and this recent paper illustrates one of them. The authors are trying to answer a difficult but important question: what’s inside mitochondria, anyway? Those little guys are of course crucial for life, and we know of a great many disorders involving them that we would like to understand better (and to fix). But taking a roll call inside them is very much nontrivial. Part of the problem is that there are many proteins (and other substances) that have fractions both inside and outside the microchondria, with no firm guarantees that they’re doing the same things in both compartments. Another headache is that the mitochondria themselves have some pretty specific zones - the mitochondrial outer membrane, the inner membrane, the distinct space between those two, and the matrix on the inside once you’re past all those other zones. There are all sorts of transport proteins policing these layers, moving stuff in both directions under different cellular conditions, so it’s a complex and dynamic environment.

In some cases, you can look at protein sequences and find “targeting sequences”, short stretches of amino acids that interact with such transport proteins to help sort things out. Previous work by these authors, though, had shown that there are plenty of proteins that are localized to the mitochondria that don’t have any of the known targeting sequences, along with plenty of proteins that do seem to have them that don’t seem to be imported into mitochondria! So you can only get so far by looking for these localization sequences.

This most recent paper uses “split-reporter” technology, which takes advantage of a property that some proteins have where they can be basically broken into two pieces that are capable of reassembly into a functional whole under cellular conditions. Here they do this with a Green Fluorescent Protein, and GFPs are of course absolutely workhorses of chemical and cellular biology research for their detectability. This team breaks the GFP protein into a large fragment and a small one, and takes advantage of the way that mitochondria have their own localized DNA (a remnant of the long-ago time when they were free-living organisms, before becoming symbiotic with other cells). DNA coding for the large GFP piece is inserted into the mitochondrial sequences, while the proteins under investigation all get tagged with the small piece. By this method, the only green fluorescence will be when one of those proteins shows up inside the mitochondria, where it will recombine into a functional GFP and shine a green beacon at its location.

Since it’s the days of modern chemical biology, the authors did this on the entire yeast proteome, tagging everything with the small-GFP sequence. Meanwhile they generated a yeast strain that had the “big GFP” incorporated into its mitochondria. Taking the all-labeled-protein cells and depleting them of their mitochondrial DNA, then immediately allowing them to mate with the big-GFP strain produced a generation that had both sides of the split-GFP system in the same yeast cells. They then imaged these under a wide variety of growth conditions (different food sources and different sort of cellular stress).

Interestingly, those food-and-stress variation didn’t seem to yield much - the mitochondrial results all looked pretty similar without signs of lots of different proteins being imported under different conditions. And a lot of the proteins that showed up were already known to be mitochondrially localized (which is a good thing - if those hadn’t shown up it would have set off alarm bells that something was wrong with the method in general). But they were able to pick out 56 new proteins that had never been imaged in mitochondria before, 39 of which also show up in other regions of the cell (that problem mentioned earlier). Eight of them were never known to be associated with mitochondria at all.

As the authors note, their C-terminal tagging is certainly capable of missing some proteins, although it’s hard to say how many. The method seems to have good sensitivity, so they’re more worried about misses due to problems with the labeling than about misses due to low abundance, at least for now. One weird thing is that five of the eight new annotations turn out to be ribosomal proteins, and you wouldn’t expect those to have any real functions inside mitochondria (!) Their properties, to be sure, do seem to be more like other mitochondrial proteins than your average nonmitochondrial ones, so there could well be something new going on here, but who knows what! More experiment will be needed to figure that out. But one of the other results does inspire confidence: a protein called Gpp1 was found in the mitochondrial set that no one had ever annotated that way before, and the authors confirmed that there area actually two different transcriptional isoforms of it (a fact not appreciated before) and that one goes to the cytosol and one to the mitochondria. Why, and what it’s doing there, will be the subject of future work!

But overall, this paper illustrates the amount of work that had to go into this localization research, and the amount of cross-checking needed to make sure that you can believe your own results. People are chipping away at this sort of thing all over the cell and adding to our knowledge all the time - because, as I like to point out, you certainly can’t go ask an AI model about these things and get any meaningful answers. . .

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Posted by Ask a Manager

It’s “where are you now?” month at Ask a Manager, and all December I’m running updates from people who had their letters here answered in the past.

There will be more posts than usual this week, so keep checking back throughout the day.

Remember the letter-writer asked whether they’d been wrong not to interview a problematic volunteer for a paid job? Here’s the update.

You and the commenters were extremely helpful. I was reassured that the decision itself was not inappropriate, but better communication would have helped a lot along the way (isn’t that always the case?).

One of the first things I did was go back and re-read the personalized rejection I had sent Stephanie after the second interview. I definitely overcompensated and was way too complimentary of Stephanie and her skills, when ideally I would have gently closed the door. I completely own that and recognize that I did not manage expectations correctly. We also found that she was latching on to a comment our director made about one of the skills we were especially looking for that hiring round. Stephanie felt we didn’t adequately emphasize that skill in the job description and give her a chance to talk to us about her possessing that skill. It was a mistake for us to have suggested that skill was related to her rejection — it wasn’t — and it ended up adding to her perception of unfairness. None of that excuses her subsequent behavior, of course, and, realistically, probably wouldn’t have prevented it.

The commenters also honed in on Stephanie’s racist comment about our staff member being a diversity hire. Trust me, I was beyond done with Stephanie as soon as I heard that she had said that, and it made it hard for me to want to extend any compassion toward her hurt feelings. Several commenters suggested that she should have been relieved of her volunteer duties as a result, and in my dream world that would have happened. But the reality is, it’s hard to use what she said as a fireable offense, even though we all know what was behind it. (I have a lot of feelings on this topic, but that’s a whole other post.)

Stephanie claimed she “could act like an adult” and continue volunteering. That’s … debatable. She remained pouty, seething, and would barely look at or speak to me and our director. Being in the same space with her was uncomfortable. There was so much anger and awkwardness coming from her that I didn’t think having an extra conversation with her about why she wasn’t selected was the right move. (For a different candidate, that certainly could have been for the best.) Stephanie eventually dropped one of the weekly tasks she was doing and scaled back on her volunteer efforts overall. I rarely see her anymore, and when I do, she is formal but pleasant.

Due to some recent internal promotions, we actually posted a job opening again this fall. Stephanie did not apply. She did, however, choose a day and time when I am usually in the office to come in and announce to us all in-person that she would not be applying this time. I think she wanted us to understand that it isn’t because she thinks she isn’t qualified, but rather because she neither needs nor wants us. Unfortunately, and by total coincidence, I was out of the office that day and missed her big reveal.

The post update: were we wrong not to interview a volunteer for a paid job? appeared first on Ask a Manager.

#54: N.D. Stevenson, Nimona [JRI]

Dec. 18th, 2025 12:18 pm
kareila: a lady in glasses holding a stack of books (books)
[personal profile] kareila posting in [community profile] kareila_books
I've had this on my TBR pile since purchasing the graphic novel at Powell's ten years ago, but that copy is still gathering dust on my shelf. Instead I listened to the audiobook adaptation today while working through some ill-considered seasonal gift knitting. It's sweet and poignant, with more emotional depth than I expected from the premise.
[personal profile] tcampbell1000 posting in [community profile] scans_daily


JLI #16-17 introduced the Queen Bee and her alliance with Jack O’Lantern. In that first appearance, she was all poise and grace. Despite her chilling games of mind control, she also exuded a false warmth that snared lovers and allies and disarmed her enemies.

In her second appearance, the warmth is gone. It’s true what they say: holding high political office ages people before their time.

But why won’t certain office-holders DIE of old age already? )

my favorite posts of 2025

Dec. 18th, 2025 05:29 pm
[syndicated profile] aam_feed

Posted by Ask a Manager

Here are my favorite posts of 2025, in no particular order:

1. my employee keeps insisting he looks much younger than he is (but he doesn’t)
Because humans are weird, and I love that.

2. good things that came from socializing with coworkers: marriages, dog adoptions, and more
Because this was heart-warming, and these things are easy to overlook.

3. how much deference do good managers want from employees?
Because breaking down this kind of question is one of my favorite things.

4. my team doesn’t want to work for a client whose politics they disagree with
Because a lot of people are grappling with this right now.

5. my colleagues are upset that we’re not “speaking truth to power” on social media
This one too.

6. my new team thinks they’re incredibly overworked, but they actually do nothing
Because this is a fascinating mystery.

7. does HR-mandated manager training ever fix bad bosses?
Because this question lurks in the background of so many other questions here.

8. will I offend my coworkers if I invite them to my religious wedding?
Because I learned a lot from this letter.

9. my boss loves being told she’s beautiful
Because this is hilarious.

10. the unflattering photographs, the cat medicine, and other times people used their power for good
For obvious reasons.

This was also the year we added the sagas tag for letters with multiple updates!

The post my favorite posts of 2025 appeared first on Ask a Manager.

oursin: My photograph of Praire Buoy sculpture, Meadowbrook Park, Urbana, overwritten with Urgent, Phallic Look (urgent phallic)
[personal profile] oursin

Trust's £330k appeal to buy Cerne Giant's 'lair' - if anyone is unaware of the existence of the Cerne Giant, I should issue a NSFW warning for the images - 'the ancient naked figure sculpted into the chalk in Dorset' with a gigantic todger.

The trust said purchasing the land would allow the charity to restore and care for sections of chalk grassland, plant new woodland, and create habitats to support species under threat.

Well, we think there is some primeval fertility mojo all ready to support the threatened species, no?

The National Trust has looked after the Giant and the immediately surrounding sward since 1920. (I now want to poke about in the British Newspaper Archive to see what the reporting, if any, was like....)

And in related matters of burgeoning nature and the work of the National Trust, More than 300 seal pups have been born at a colony just a month into the breeding season:

Last year, 228 pups were born at Orford Ness in Suffolk, which is home to the county's first breeding colony of grey seals.
The breeding season began in November and already hundreds have been born with still about a month to go.
Matt Wilson, the trust's countryside manager, said the team believed the entire colony now consisted of more than 1,000 seals.

***

And another form of conservation: The Digital Future of Stained Glass: Data Standards and Interoperability – Why Recording Stained Glass is Important. (What this sounds like to me is a whole lot of people not talking to one another while doing very similar work and only now getting together....):

Existing data however is currently presented in wildly different formats across different databases, to varying degrees of detail and accuracy, and held on disparate websites managed by individuals. This means that the future of these resources collectively is highly insecure.

Screaming in archivist been there and done that.

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Posted by Sumana Harihareswara

I know, from personal experience, that it takes a significant amount of effort to research, write, revise, and submit a decently plausible funding proposal to the US government's National Science Foundation. A successful NSF proposal …
umadoshi: (Yona-hime 01 (snarfles))
[personal profile] umadoshi
"Yona of the Dawn Gets Sequel Anime". [Anime News Network]

I'm delighted both that this is happening and that it was announced so promptly on the heels of the manga ending. (;_;) As we learned from the second Fruits Basket anime arriving thirteen years after that manga ended, anything is possible, but it's sure nicer to have this sort of thing happen with a speed that makes more sense.

ANN says "sequel anime", which I'd imagine means it'll pick up where the first one left off, but how OAVs factor into that, I'm not even going to try to guess.
[syndicated profile] aam_feed

Posted by Ask a Manager

It’s “where are you now?” month at Ask a Manager, and all December I’m running updates from people who had their letters here answered in the past.

There will be more posts than usual this week, so keep checking back throughout the day.

Remember the letter-writer who thought one of their employees might be trans and was wondering how to signal support (#3 at the link)? Here’s the update.

Thank you for publishing my letter in July. Your advice and the comment section were both very useful. Everyone was very kind and a lot of people had good advice.

I decided to follow the advice of not saying anything to Jane or focusing particularly on her, instead turning my focus to making work a safe environment for anyone. I also didn’t go back to the YouTube channel, figuring that Jane had a right to keep her private and professional life separate. Not to mention, I didn’t feel comfortable going into the comments section to say “Hi this is your manager” and being a silent follower, or commenting without her knowing who I was, felt too close to stalking. At the end of the day, if I started to stream outside of work as a hobby, I don’t think I’d want anyone at work to watch, much less someone I report to. And if I want to watch streams of video games, I’ve got more than enough choice without having to watch this channel in particular.

Jane didn’t end up coming out, but another employee did, about a month after I wrote — and, funnily enough, around a week after my letter was published. To keep with the Disney names theme, let’s say Eric came out as Ariel, a trans woman. I made it known publicly that I wouldn’t tolerate any discrimination towards her, and that anyone under my supervision who gave Ariel a hard time would answer to me. I also started educating myself on gender identity; I had started before this happened, but I can’t lie and say it didn’t motivate me to spend more time on it. What was a vague possibility — managing a trans person — was suddenly an immediate reality.

The good news is, our team really was as open-minded as I hoped they would be. It took some time for everyone to get used to the new name and pronouns, but they were all gracious when Ariel corrected them if they slipped up, and at this point no team member is slipping up anymore. One person did try to ask, within my earshot, if Ariel was considering bottom surgery, then looked horrified when I asked if I’d heard them inquire about a coworker’s genitalia. I hope the question was born out of misplaced curiosity rather than actual malice, but either way I knew I had to shut that down. As I said in a comment on my first letter, as far as I’m concerned my coworkers might as well be Barbie and Ken dolls with no genitalia. I don’t want to know, and I won’t have my team trying to know either. Luckily, shutting it down once and mentioning that this could be grounds for a sexual harrassment complaint was enough, and it didn’t happen again. I made sure to mention to Ariel she shouldn’t feel obligated to answer such questions, and she was free to come to me if something like it happened.

The bad news is, the rest of the company wasn’t so great. (And yes, I know several people said that even if my team was open-minded, everyone might not be; congratulations, or condolences, you were right about that.) Nothing was done that could give Ariel grounds to make an official complaint about discrimination, because the law was followed to the letter … but not so much to the spirit.

We all wear company-issued uniforms, and despite a lot of push back, that uniform is still partly gendered. Women are allowed to wear skirts or trousers, but men have to wear trousers. They refused to give Ariel a skirt until she legally changed her name and gender. Until she did, they were (legally) allowed not to count her as a female employee. Similarly, she still had to introduce herself as “Mr Eric X” on the phone and in her signature, because they wouldn’t switch her info until the legal change was made (which, in our country, can take a month to over a year, depending on where you live).

I pushed back against this as much as I could. I insisted the spirit of the law matters just as much, if not more, as the letter. I also offered a sympathetic ear to Ariel when she felt the need to vent about this whole process. Our team also rallied behind her and offered support. It took multiple complaints to HR, as well as people “casually” commenting in front of higher-ups that they didn’t think our company was so backwards, and they might have to consider looking for a new job that aligned more with their values, to make the process go smoothly. Ariel finally received her new uniform and was allowed to introduce herself as her real identity, as should have been the case from the beginning.

Unfortunately, I can’t say the lesson was learned, since now the company is trying to use Ariel as an example of how inclusive they are, in a “look, we have an openly trans woman working for us” way. In fact, the former head of HR had the gall to say in their retirement speech, “I’m proud to have worked for a company that accepted Ariel, a trans woman, with open arms” or something to that effect. Everyone in the audience was extremely uncomfortable, none of us more than Ariel, of course. On another occasion, a new employee was being introduced to everyone and when it was Ariel’s turn, boss said, “And this is Ariel, our very own trans lady.” This was met by immediate outrage from the team, and I pointed out that one, people aren’t minority tokens (just like you wouldn’t say “this is our very own BIPOC employee”), two, this was objectifying as it implied Ariel belonged to us, and three, he had just outed her without consent or warning. While he made a show of apologizing, I later got informally reprimanded behind closed doors for undermining him. I still think calling him out on the spot was the right thing to do, and all the reprimand told me was that my boss didn’t actually get what he’d done wrong. I reported the incident to HR, but as far as I know nothing came out of it. It is, of course, possible that my boss got a talking to or a warning and I wasn’t told about it, but it doesn’t seem likely.

It’s not a perfect update by any means (I’m not even sure it qualifies as a good update) and I know the entire situation has led Ariel to reconsider working for this company. If she does find a new job somewhere else, I’ll be sad to see her go as she’s a very competent worker and a very nice person to work with, but I can hardly hold it against her. I’ll be happy to provide her with a glowing recommendation if she ever needs one, and I’ve told her that. I’ve been updating my resume myself, though I won’t be looking to leave just yet; I want to be here to support Ariel as long as she stays with us.

Thanks again to you, Alison, and to everyone who commented with advice on how to be more inclusive and handle my initial situation.

The post update: I think one of my employees might be trans — how can I signal support? appeared first on Ask a Manager.

THE FINAL COUNTDOWN

Dec. 18th, 2025 02:00 pm
[syndicated profile] cakewrecks_feed

Posted by Jen

Just one week left 'til Christmas (!!), and the wrecks you guys are sending in just keep getting more bizarre by the day:

Snowman? Santa? Disco sheep? Marshmallow-covered Walter Peck?

  

THE WORLD MAY NEVER KNOW.

"...and all the acid rain and pollution seeped into Frosty's body, turning him into a petrified statue of ash, all because someone didn't take out the recycling when his parents asked.  

"THE END."

 

 Is this:

A) The Eye of Sauron 

B) A taco wearing a corset 

C) The Eye of Sauron wearing a corset

or 

D) Real life?

 

You might think the simplest, easiest option would be an elegant red bow on a plain white cake.

But, CLEARLY, you would be wrong.

 (What did the baker use for a piping tip? His teeth?)

This next one is tentacly titled "I'm dreaming of a Cthulhu Christmas."

Darkly dreaming, of course.

 

Wondering what those are supposed to be? So was I, until Sarah sent in this photo from a different store:

Now I'm REALLY curious.

 (Yes, yes, I know it's supposed to be a tree. I just refuse to accept it.)

 

And finally, a little holiday math for you:

+

=

Angels we have heard below

Softly singing "oh-hh noooOooo!"

 

Thanks to wreckporters Elizabeth T., Tina, Gene H., Jill W., Billy G., Sarah L., & Amy V. for giving Sharyn a starting point for today's song re-write in the comments. (Challenge: ISSUED.)

******

P.S. I've been shopping for the best Christmas lights to hang outside this year, and I think you'll like what I bought:

BrizLabs Color-Changing Christmas Lights

This is a 115 feet of LED lights, which you can change from warm white to multi-color to any combination of the two! They have 11 different settings like "slo-glow" and "breathing," a remote control, and even built-in timers. All for $27! This is the best price for the most features I've found, plus they have great reviews, which is a must for me.

John & I installed 3 strands of these beauties last weekend, and they. are. GORGEOUS. Highly recommend.

*****

And from my other blog Epbot:

Thankful Thursday

Dec. 18th, 2025 04:05 pm
mdlbear: Wild turkey hen close-up (turkey)
[personal profile] mdlbear

Today I am thankful for...

  • The Unreasonable Effectiveness of Mathematics in the Natural Sciences. (See also, the Wikipedia article, Watch out for the rabbit hole -- this is a deep one.
  • Mail arriving in time (though just barely). Don't count on UK's Royal Mail being as fast and consistent as Postnl.
  • Receiving packages that I feared had gone astray. Looking deeply enough into them to realized that, in addition to failing to provide my house number on one order, I had mixed them up because their package numbers had the same last digit.
  • Nanobag and Roamate. (See above.) (I want to review the latter eventually. However, the best-laid plans, etc.)
  • Not sure how thankful to be for decade-old scratch tracks, but they deserve a listen at least.

2025 Diamine Inkvent Teal - Day 5-8

Dec. 18th, 2025 03:00 pm
terriko: (Default)
[personal profile] terriko
This is crossposted from Curiousity.ca, my personal maker blog. If you want to link to this post, please use the original link since the formatting there is usually better.


Second set of Inkvent inks! Yes, I’m behind. It’s been an absurd month as we prepare to move, with our entire plans getting derailed repeatedly, but most of this week’s news has been good so here’s hoping!





Swatches from day 5-8 of the Diamine Inkvent Teal (2025) calendar. The colours are named Marie Rose, Fir & Fob, Blush and Dream Catcher.




Day 5: Marie Rose. Kind of an orange-brown that shows a bit of black at the edge of the bigger swatches. I wondered why it was names “rose” when at best it might be a really desiccated old rose petal colour, but apparently it’s named after a type of sauce and not after the plant. I actually don’t have anything particularly close to this colour and it’s a nice uncomplicated standard ink so it’ll definitely get used even though it’s a colour I might never have thought to buy.





Day 6: Fir & Fog. Beautiful blue-leaning green with iridescent shimmer. It makes me think more of spruce than fir trees but I don’t think most people think that deeply about their evergreens. Love it. I’d probably get this even without the shimmer; it’s a really nice colour. Plus the name really makes me feel like Pacific Northwest winter, where I see the big Douglas Firs looming out of the fog many mornings.





The same Diamine Inkvent Teal day 5-8 swatches shown with the camera at a different angle to show shimmer & sheen better.
The same Diamine Inkvent Teal day 5-8 swatches shown with the camera at a different angle to show shimmer & sheen better.




Day 7: Blush. Pink. This one goes on the page looking like fresh nosebleed (look, I was one of those kids and I’m very familiar with what a drop of fresh blood looks like on the page) but it dries pleasantly into a red-leaning pink. I think the closest colour I have is Pilot Iroshuziku Momiji which is more pink and less red. I’ve been struggling to use Momiji in palettes because it’s a bit too bright, so I think the slightly more muted feeling Blush is going to fit in really well.





Day 8: Dream Catcher. There is so much pink sheen that you can barely see the base colour, but it is actually blue. This is fun but *very* similar to last year’s Cosmic Glow, so even though it’s lovely it maybe wasn’t quite as exciting as it would have been if I didn’t have last year’s colour. I had to really stare at them together to see that Dream Catcher’s base blue is subtly more greenish than Cosmic Glow’s (but it’s not as far over as Vibe, also in last year’s inkvent). I have a few other similar blue-with-pink-sheen inks but none of them have as much sheen as these so they feel pretty different.





Dream Catcher and Cosmic Glow swatches showing that they have the same colour of pink sheen but slightly different base colours.
Dream Catcher and Cosmic Glow swatches showing that they have the same colour of pink sheen but slightly different base colours.




Dream Catcher and Cosmic Glow swatches shown at an angle so the sheen is less visible and you can see that they have slightly different base shades of blue, with Dream Catcher leaning slightly more greenish
Dream Catcher and Cosmic Glow swatches shown at an angle so the sheen is less visible and you can see that they have slightly different base shades of blue, with Dream Catcher leaning slightly more greenish.





Even though Dream Catcher is nearly a dupe and a colour family that’s already over-represented in my collection, these are all very pretty and will definitely get used. If I had to guess, Blush is the one that’ll get used most often because I have wanted a different pink for a while, but I’m really in love with the spruce green of Fir & Fog plus the name will make me think of the pacific northwest winter.

The Merro Tree by Katie Waitman

Dec. 18th, 2025 08:46 am
james_davis_nicoll: (Default)
[personal profile] james_davis_nicoll


A determined artist faces potentially lethal criticism.


The Merro Tree by Katie Waitman

(no subject)

Dec. 18th, 2025 09:41 am
oursin: hedgehog in santa hat saying bah humbug (Default)
[personal profile] oursin
Hasppy birthday, [personal profile] nomeancity!
amberite: (me)
[personal profile] amberite
So, despite having not a lot of money, I've lately been able to get a ton of random stuff I've wanted. Small electronics, art supplies, home organization supplies, more different kinds of purple clothing than I imagined existed - you name it. (The main limitation is that our apartment is very small.)

This is because earlier this year I got on Temu to buy some business supplies, mostly in the interest of divesting from Amazon. Now they are giving me a deal where, if I spend $200 in a sitting, I literally get the entire price of my purchase refunded except the sales tax and sometimes shipping (but not inflated shipping! That would make too much sense!) And then sometimes they don't manage to ship me the items in time so I get credit for delays, which covers the sales tax. It's kind of absurd. 

Why this is happening, I have several theories. I'll share them here, in the order of "most similar to mundane economic activity" to "kinda wild but OK."

I suspect multiple of these are true to some extent.

1. Maybe most people fail to complete the rebate process correctly. The process is rather fiddly. If you miss logging in for a day, you lose a big chunk of the money back. If you order less than $200 at a time, you don't get the full rebate. At that point, you are paying for regular discounted goods, a decent deal but nothing special. 

....BUT I'm completing the process correctly, and they keep giving me the rebate, so that can't be the whole story. (Also note that previous Temu deals have been known to kick people out of the promotion eventually if they claim too much of the money successfully.)

Very well, more theories:

2. This is the equivalent of a brushing scam, without the scam. The algorithm has figured out that I leave useful, honest reviews and leave a lot of them, so they're sending me free shit in the knowledge that I'll likely respond, naturally on my own, by improving the credibility of the platform. This certainly might explain why they're still giving me the rebate deal despite my reliability at claiming the money. 

3. Temu is trying to inflate its Q4 sales figures. There are many reasons why this could benefit them - investment, taxes. 

4. Temu is engaged in some form of money laundering. What form and why, I got nothin'. (Well, okay, I got a wetsuit, a tattoo gun, and a lifetime supply of 2gal plastic ziploc bags.)

4b. The Chinese government is throwing money at Temu, which in turn is throwing it at its customers. This works reasonably well in concert with 3 or 4a.  The motivations could be: undercutting Amazon, establishing monopoly, spiting Trump over the tariffs, or - and I'd bet it's at least a little bit this, because it's the right style of "communism-capitalism cookie sandwich" for them - ensuring the manufacturing economy continues to keep workers employed. 

Anyway, now that I've established that they really are reliably sending my money back & I have most of the fun things I want, I'm ordering useful stuff. This has its own hilarious economic caveat:

- Most of the brand-name practical expendables on Temu are actually drop-shipped from Walmart, Target or Amazon. 

You know how you used to sometimes buy stuff from a US web storefront and find it was actually shipped from a random Chinese seller? Well, now they're doing the opposite. The telltale signs of this are that the item ships from a domestic origin point and costs more than normal. It's harder to find these items on the platform than it is to find clothing and bling, they go fast, and I wouldn't normally order them at this price point, but... yeah, money back... 

For example, I "spent" $35 on an order containing a small box of Tampax tampons, a large box of Band-Aids, and a bottle of Neutrogena body wash. These items would have probably cost a total of $25 in the store. I ordered them knowing that I would be refunded all but the tax. Some 3rd party vendor sent me a Walmart package and pocketed the difference. 

Other things I've been ordering a lot of this way are brand-name supplements and essential oils. (I still want to start doing perfumery again someday.) 

I've also started ordering altruistically, because I'm sure this deal will end eventually and I'd like to make other people happy. One of our homeless friends down at the beach, who deserves a whole post or two on here himself - he's the one who made me realize that Venice Beach is basically a town full of urban fantasy protagonists - is always wanting to borrow my phone to play music because he can't hang onto one without getting rolled for it. I ordered him a music player and speaker. Got a big box of hand warmers and emergency blankets to give out, too.

And I've just picked up a cat carrier to donate to a rescuer who's been doing work to help us gradually resolve a friend's Infinite Kitten Hell problem (poorly educated immigrant parent adopted a bunch of strays without realizing how important it was to spay/neuter. Predictable events ensued & every vet in LA is backed up on spays, so you have to know someone.) 

(P.S. - anyone up for taking on a spare kitten or cat? My friend's family are decent people and caring for the ones they've brought into the world, but it's not really a healthy number of cats to have.) 

December Days 02025 #17: Persistence

Dec. 17th, 2025 11:30 pm
silveradept: A librarian wearing a futuristic-looking visor with text squiggles on them. (Librarian Techno-Visor)
[personal profile] silveradept
It's December Days time again. This year, I have decided that I'm going to talk about skills and applications thereof, if for no other reason than because I am prone to both the fixed mindset and the downplaying of any skills that I might have obtained as not "real" skills because they do not fit some form of ideal.

17: Persistence

As someone who is comfortable with installing and reinstalling and restoring configurations and working my way back to what it was before, just with time and scripting, and exporting and importing, it's not the end of the world when an entity or a corporation pulls a milkshake duck, or decides they, too, are going to chase the snake oil bubble and start cramming LLM-related features into their browsers, or operating systems, or any other piece of software they can control. I will freely admit that it sucks to have to do all of those operations on the regular, or even on the occasion, but it is something that I have become used to, as I've been throwing things around here and there, and making it work better. The hardest part, sometimes, is re-learning where you've stashed all your configuration tweaks and where they get applied to. But the more it gets done, the easier it is to remember where all the pathways are, and what you want to do with them. Perhaps in some future world, I'll remember to save the configuration files first, and back them up, and then retrieve and paste them back in and all will be well.

And, when I make these kinds of decisions, as it turns out, sometimes I learn some new and interesting things, like the way that some apps, even if they don't exist in the package manager, are self-contained enough to run on the system. Therefore, I now have my preferred browser running on a system that doesn't have it in the package repositories. At least, not at the moment, since the new version is built on one version up from where my current distribution wants to be.

This is also a crossover post with the Adventures in Home Automation series, because, for the third time, I have managed to get my television with the attacked Raspberry Pi and the broken IR receiver talking to Home Assistant, and being controllable from there. In the previous incarnations of this situation, I managed to clone some git repositories, recognize that some of the things they wanted to do with containers and running the thing as they would like to wouldn't work, because they were asking for some much older versions of Debian, which were probably the newest versions of Debian at the time, but whose archive pointers had completely fallen off and were no longer available. One promising entity written in go worked for a little while, and then the go language changed versions, and the old script just went "nope" compared to the new version, and I don't program in go, so I couldn't fix it. The second promising entity was written in python, and in a previous version of Debian, I seemed to gather all the right libraries from the system tools and get very close to making things work, before I dropped a piece from a completely different script, meant to make it possible for a remote control to function as a game controller, I believe, into the other script, because it looked like it might work. And it did, to my surprise. So that was version two, running stably and with a systemd service for running on boot, happily working its way along.

Then the Debian version underlying the single-board computer's Linux changed, and that meant not only rebasing, but reinstalling, reconfiguring, re-adding, and otherwise bringing things back into the system I had, and reinstalling and reconfiguring the communication broker so that the SBC could communicate with Home Assistant (and the router, now that it had some Optware installed that would send information about router operations and connected machines over that same protocol, using that SBC as the broker for the messages.)

The last component that needed to work was the bridging script that reported information using HDMI-CEC to read the bus for status and then transmit commands from Home Assistant to turn that screen on and off. In the intervening time, the library that the python program used to communicate had jumped a major version number and changed its entire syntax in the process. Luckily, the error that appeared mentioned that a single flag could be set so that it would use the old version of how it was set up, and that saved me a lot of grief trying to figure out how to re-spec the script to use the new library. The flag may deprecate at some point, and then I will have to walk the script up from the previous version to the current version. Hopefully, when that's necessary, there will be a nice conversion guide posted somewhere that explains what the equivalent commands are, and where to put the components of the previous command in the new syntax. For now, however, the scripts themselves are sorted, thanks to adding one piece of code at the right place to the thing itself.

What's not working is that in this new version based on Debian Trixie, the library I had installed from the earlier version was no longer present. And that meant a significant amount of looking around to see if there was something suitable that would serve in its place. The testing repository, the one that would be in the next release (Forky), had the library I thought I had installed on the previous version. So, I did something that is recommended against, and added the testing repository and pulled the version of the item from there, expecting it all to set up and go.

No dice. So I uninstalled that particular set of libraries, because pulling from different releases is a good way to break it. Option two: since it's a python script, I can potentially set up a virtual environment for Python, separated from the system-managed Python installation, then install the necessary libraries through the pip package manager to the virtual environment, and run the script out of that, so long as said script can communicate out and have Home assistant pick up what it's laying down. That's easier to manage with some software packages like pipx to handle the creation and management of the virtual environment. I get the environment set up, and the library that I think will work installed, and the script bombs again with the same error as it had before, So the virtual environment approach isn't going to work, either.

All this time, I'm using my search engine skills to try and figure out what the error is, but there aren't a whole lot of posts on the subject, and most of the time, it keeps coming back to a couple of places, including a GitHub issue that seems like it's exactly about the problem that I'm having, and that somehow the problem was fixed in a subsequent release of the software, but I don't see how they got from point a to point b, as I read and reread the information and keep trying to figure out where the library is that I need to install from the package manager to get the functionality I had before.

This is one of those things where sometimes you need to let your brain background solve a task. Humans are, after all, persistence predators, and while flashes of insight are often cool, they often come more after you have been chewing on a problem for a while, letting it background-process while you work your way toward greater understanding. There was a study, I believe it was in one of my graduate school texts, where a professor gave students a list of riddles to try and solve over the course of a day. At the lunch break, the professor collected the tests and had the students do their lunch break activities, but at places along the way in the building, the professor had placed representations of riddle solutions, and the thing that was being tested was whether the presence of those solution prompts helped the students solve more riddles. I can't find the study, and so I may not be representing it accurately, but sometimes you go through an entire something and as your brain twists and turns on it, and eventually, you do some up with something that actually qualifies as a solution to the problem. It's the idea of "distracting" your conscious processes so that some other process can take over the solving of things, or the integration of information. Sometimes sleeping on it is the right answer to the situation.

In my case, the actual solution came when I finally realized that I was making an assumption that one of the forum posts explicitly denied was a good one to make, and that instead of installing a package from a repository with a similar name, but not actually containing what was needed to succeed, what I instead needed to do was follow the instructions that were given in the right place and compile the damn library myself. Which there was definitely a recipe for, and for the specific architecture and device that I was using. Download source, pass appropriate flags to the compiler, make, make install, all of the things that are involved in compiling a library from source, and guess what? As soon as I had compiled the correct library, the script worked perfectly as I ran it, with the "use the old version please" flag set for the library that did some of the work.

I felt very stupid afterward, because everything kept funneling back to these posts that said "no, that package is not the library you need, you have to compile the library from scratch, and this is the way to do so." I didn't want to do that because I'd rather use the package manager to produce the thing that I needed, instead of compiling something from source. Actually doing what the thing said only took a few minutes and would have avoided many months of grief and not understanding why things weren't working, even with the ability to search up the specific error message and find the post that described it accurately and said what the solution was. Once I managed to read the post correctly and drop the preconception I had, things went much more smoothly.

So this is about the persistence of solving problems, of trying to get to a solution that works for me, and sometimes the disappointment that comes when someone is satisficing rather than looking for a full solution. It's about persistence, because apparently I keep wanting to tweak and shuffle and suggest and do things until they're exactly right, instead of mostly right. It's also about how that persistence sometimes means it's hard to let go of the situation if it's not perfect and optimized and works in all cases. And how it can be annoying to have to deal with people who deliberately want to keep introducing nonsensical edge cases into your perfectly working system, or who believe that if you don't debate them on their nonsensical edge cases or absurd questions, they have somehow "won" and proven themselves smarter than you, because you refused to engage with bad faith tactics. As the somewhat ineffectual advice given would tell us, we can only control ourselves, we cannot control other people. (In pursuit of perfection, we seek control, and sometimes the control that would produce perfection is the control of others, and therefore, perfection will always be beyond us. In theory, this realization is supposed to help us not seek that level of control. In practice, there's still a lot of frustration that comes from not being able to do the things flawlessly and well, and sometimes even more aggravation when things are going out of our control and we don't even know why.) Given how often I end up having to engage with the absurd and the nonsensical, I'd like to believe I have a greater tolerance for other people being Wrong on the Internet (or in my workplace), but there's still sometimes that bit where I want to believe that with enough persistence, I will be able to prevail over the things that bother me, or the people that bother me.

It's also, though, about persistence, the concept that we first learn about when object permanence makes it into our head, that the world is not, in fact, limited to what we are experiencing with our senses, and that our senses (and our minds, if you want to get Zen about it) are misleading us about the nature of our reality. Just because the ball disappears behind the paper doesn't mean it winks out of existence entirely, only to return into reality when the paper is raised. (At least, at the Newtonian mechanics level. Quanta and their friends behave very differently, and we are finding more and more that the act of observation collapses all the possibilities into an observed real, such that whatever organ we are using to perceive the possibilities with inscribes what the result will be onto those possibilities.) The past and the future are constructions, only Now is reality, and only for the now that we experience Now. Many of those constructions are useful, and society rests on our ability to construct things about past, future, and pattern so that we can attempt to impose some amount of order upon the chaos, so as to make it livable and manageable. (That's karma, baby.) We persist in things all the time. Error. its opposite. The horrors persist, and so do I (or but so do I.) Nevertheless, she persisted. He's baaaack! So many things that we have in our history and our lives are about the application of human-sized amounts of influence and force until the desired result is achieved, sometimes even with a great array of things standing athwart, sabotaging, or attempting to cause failure in the way. Because we are not the kinds of beings that let go easily, or give up, and we do much greater work when there are more of us, so we can each take a turn at persistence while someone else rests up for their next turn. The idea about the arc bending toward justice is not a thing that happens by itself, it happens because there are people bending the arc into the desired shape. We will not complete the work in our lifetime, but neither are we excused from doing the work during our lifetimes. And through the ages, thanks to our persistence, we build and sustain things that are greater than any one person and one lifetime. (It's frustrating not to see when it finally clicks into place, but ours is not to know the day or the hour, apparently.)

Only a little while longer, and some of the decisions that I made in the past, decisions that were absolutely correct, will finally have discharged their consequences. It always seems impossible until it is done. Keep at it.

Just One Thing (18 December 2025)

Dec. 18th, 2025 08:41 am
nanila: me (Default)
[personal profile] nanila posting in [community profile] awesomeers
It's challenge time!

Comment with Just One Thing you've accomplished in the last 24 hours or so. It doesn't have to be a hard thing, or even a thing that you think is particularly awesome. Just a thing that you did.

Feel free to share more than one thing if you're feeling particularly accomplished! Extra credit: find someone in the comments and give them props for what they achieved!

Nothing is too big, too small, too strange or too cryptic. And in case you'd rather do this in private, anonymous comments are screened. I will only unscreen if you ask me to.

Go!
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nataliedecorsair:

nataliedecorsair:


The collection of my Mari Lwyd art through the years

First art is called: “Dude… give me the bottle” (or when you lost a rhyming contest against the supernatural skeletal horse)
Second art is a meme reference x)
Third art is featuring Mari on her day off (hence no rhyming, but she wouldn’t say no to a treat)

Plus other stuff, silly sketches and artworks, like Mari Lwyd in modern times being disappointed that nobody wants to rhyme with her anymore, so she has to buy everything herself (featuring a distant relative of the guy from the first piece, plus a Waterboy mashup).

P.S. - don’t forget about the frogs in some of the arts

Mandatory winter reblog of my Mari Lwyd post, now updated with new artwork 💅
December is my birthday month (in the end of it), so I’m very happy to have such a wonderful cryptid on my side

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